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PCR Technique with its Application

Kavya SR

PCR (Polymerase Chain Reaction) is a revolutionary method developed by Kary B Mullis (awarded Nobel Prize for chemistry in 1993) in the 1983. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the template strand of DNA. This technique is having an impact on many areas of molecular cloning, genetics, recombinant DNA research molecular biology, forensic analysis, evolutionary biology, and medical diagnostics.