Sunil J Panuganti
Bioassay is defined as estimation or determination of concentration or potency of physical, chemical or biological agents by means of measuring and comparing the magnitude of the response of the test with that of standard over a suitable biological system under standard set of conditions. Bioassay is a successful tool in estimation and discovery of biologically active substances and important application in sensitivity and specificity of pharmacological applications. Chemical method is very complex method which requires high chemical dosage and chemical composition shows the pharmacological actions against the drug.