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Adventitious shoot regeneration in Sesame (Sesamum indicum L.) (Pedaliaceae) via deembryonated cotyledonary explants

Shilpa V. Malaghan, Ramappa Lokesha, Revadi Savitha and Ajjanavara R. G. Ranganatha

Adventitious shoot regeneration in sesame, via de-embryonated cotyledonary explants, was attempted using six diverse genotypes; two were land races (Kankapura Local – KNL and Tamil Nadu Local- TNL), three were released varieties (DS-1, DSS-9 and WII) and a breeding line (RT-273). Three media compositions (½ MS basal media supplemented with 20 μM TDZ + 2.5 μM IAA and with or without 25 μM BAP and full MS basal media supplemented with only 25 μM BAP) were used of which highest shoot regeneration (100%) with 8.15 and 7.37 shoot numbers per cotyledon was achieved in RT-273 in both the combinations (½ MS with 20 μM TDZ+ 2.5 μM IAA+ 25 μM BAP or without BAP) followed by DS-1(100% and 95.55% with 6.15 and 3.89), KNL (88.88% and 7.55) and TNL (88.88% and 5.73) respectively in ½ MS with 20 μM TDZ+ 2.5 μM IAA+ 25 μM BAP. MS with 25 μM BAP alone failed to induce shoot regeneration.