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Activity of Acetylcholinesterase (AChE) in OxycarenusHyalinipennisCosta (Hemiptera: Lygaeidae, 1847) After Exposure to Chrysanthemum Indicum l. ExtractRupture

Muhammad Abubaker Hassan, Shoaib Freed

Oxycarenus Hyalinipennis Costa (Hemiptera: Lygaeidae) is serious pest of Gossypium Hirsutum L. which is commercially grown to generate foreign exchange reserves for Pakistan. Both adult and nymph causes reduction of oil content, weight loss of seeds, germination rate and stains cotton with pink color during ginning. The main objective of the study was to check the effect of Chrysanthemum Indicumflower extract on the activity of acetylcholinesterase (AChE) in O. Hyalinipennis. The adults of O. Hyalinipennis were exposed to different concentration (8, 10, 12 and 14 mg/20 ml) by leave dip method prior to biochemical analysis of surviving bug samples. The results demonstrated that C. Indicum extract affect the activity of AChE in O. Hyalinipennis adult. Maximum enzymatic activity was 0.544 a-Na µmol/min/ mg protein at day 1 in T3 (12 mg/20 ml) treatment while in control maximum activity was 0.160 a-Na µmol/min/ mg protein at day 4 whereas highest and lowest mortality was (66.66 and 0%) at 14 and 8 mg/20 ml concentrations respectively. The rise in activity of AChE is one of the main reasons to develop resistance in insect. This can be managed by adaptation of alternative controlmethods.