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Extracellular Synthesis, Characterization and Antibacterial Efficacy of Silver Nanoparticles with Pink and White Nelumbo Nucifera Flower Extract.

Brindha Durairaj, and Thiyada Damodaran Vaishnavi

To synthesize silver nanoparticles using Nelumbo Nucifera white and pink flower extracts and to evaluate their antibacterial efficacy againt Escherichia coli, Bacillus subtilis and Staphylococcus aureus species. Methods: Silver nanoparticles were synthesized with 10-3M AgNO3 solution. The characterization of the nanoparticles was achived using UV-Vis spectrophotometry and SEM. The antibacterial activity was determined by Disc diffusion method. Results: The SEM analysis showed the average particle size of 60 nm with spherical shape. Further, the silver nanoparticles showed antibacterial activity against E. coli, B. subtilis and S. aureus. Stable synthesis of silver nanoparticles was investigated through this bioreduction method. Conclusion: This study revealed that silver nanoparticles synthesized with N. nucifera could be used as natural, renewable and low cost biological reducing agents, which could produce metal nanostructures in aqueous solution at ambient temperature, avoiding the presence of hazardous and toxic solvents.