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De novo Transcriptome Sequencing Based Identification of Amb a 3-like Pollen Allergen in Common Ragweed (Ambrosia artemisiifolia)

János Taller, Kincső Decsi, Eszter Farkas, Erzsébet Nagy, Kinga Klára Mátyás, Balázs K olics, Barbara Kutasy and Eszter Virág

The complete coding sequence and putative signal peptide of an Amb a 3 isoform was identified from a transcriptome dataset of A. artemisiifolia. Comparison with the known protein sequence of the Amb a 3 allergen and in silico allergenicity analyses was performed. Since Amb a 3 was known just by an amino acid sequence, the presented results contribute to explore genetic variability and expressional features of Amb a 3, as well as may contribute to immunological studies of this pollen allergen.