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Study of FLT3 Gene mutations in Acute Myeloid Leukemia

Nischala T

ABSTRACT The prognostic relevance of FLT3 D835/I836 mutations (FLT3-TKD) in cytogenetically normal acute myeloid leukemia (CN-AML) remains to be established. The constitutive activation of FLT3 receptor resulting in the worse disease-free survival in atleast some studies have been resulted by alteration of D835. D835 mutations have been reported to occur in 7% of patients with AML, 3% of patients with myelodysplastic syndrome (MDS), and 3% of patients with acute lymphocytic leukemia. By performing PCR and RFLP in 10AML tumor samples and 10 samplaes of healthy individuals of a similar age range resulted in detection of polymorphisms in the FLT3 gene mutation at 835 position. In the given study group only one case showed the homo mutant condition. D835Y (point mutation) accounts for ~8-12% of all AML patients with 113bp.The significance of the samples were not determined because of the small sample size. In this study we have demonstrated that polymorphisms (SNP’s) may play a role in cancer progression. Further studies are needed for significant results