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OPTIMIZATION OF CARBON SOURCES, TEMPERATURE AND pH FOR NITRATE REMOVAL IN SYNTHETIC NITRATE RICH MEDIUM USING BACILLUS SP. (SW-59)

Seenivasagan, R., Rajakumar, S. and Ayyasamy P.M

The efficient nitrate reducing bacterial species was isolated from water sample collected from Salem district of Tamil Nadu, India. The bacterial strain Bacillus sp. (SW-59) selected as potential organism and it showed a better nitrate removal efficiency in the nitrate reduction test. Influence of various carbon sources, incubation temperature and pH on nitrate removal was studies in mineral salts medium (MSM) supplemented with 100 mg/L of nitrate. Efficient nitrate reduction of 84.02% was attained in MSM amended with 0.5% of starch at an optimum temperature of 35°C and pH 7. This observation has led to the conclusion that the bacterial strain (SW- 59) showed better efficiency for nitrate removal from synthetic nitrate-rich wastewater