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Isolation of Nostocales and Study the Effects of Scytonemin on Growth Profile and Melanogenesis by using Melanoma Cell Line

Pranay Abhang, Girish Pathad

The purpose of this study was to screen scytonemin, a UV screening pigment, in isolated Nostocales, Scytonema sp., Plectonema sp., Spirulina sp., and Lyngby sp. Scytonemin was isolated by silica gel column chromatography and its effects were tested in vitro for growth profile and melanogenesis on B16F10 mouse melanoma cell line. In order to study effect of these scytonemin on growth profile- cell viability count, MTT and DCPIP cytotoxicity assay, Comet genotoxicity assay and microscopic observations were considered. To study tyrosinase enzyme activity- diphenolase assay and native SDS PAGE methods were used. Scytonemin isolated from Scytonema sp. and Lyngby sp. shows decrease in melanogenesis, which inhibit tyrosinase activity, having 30.25 μM, 100.25 μM IC 50 values respectively. While that of scytonemin isolated from Plectonema sp. and Spirulina sp. was not showing effect on melanogenesis as tyrosinase activity not altered, having 45.5 μM, 60.5 μM IC 50 values respectively