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Influence of Biosurfactant in Biosynthesis of Silver Nanoparticles by Pseudomonas Aeruginosa AMB AS7

Siva sankara pandian G, Mariaamalraj S, Anant Achary

The present study demonstrates green process for the synthesis of Silver nanoparticles (AgNPs) by bacteria Pseudomonas aeruginosa AMB AS7 and the role of biosurfactant in enhancing the stability of AgNPs. An aqueous solution of silver nitrate was treated with both cell free broth and biomass of P. aeruginosa AMB AS7 which resulted in the synthesis of AgNPs. The nanometallic dispersions were characterized by surface plasmon spectrum measuring at a range of 280 to 700nm. The particle distribution analysis and Scanning Electron Microscopic (SEM) results showed the formation of AgNPs in the range of 30 – 80 nm. Further analysis carried out by Fourier Transform Infrared Spectroscopy (FTIR) showed the presence of functional groups from proteins and biosurfactant. The stability of AgNPs synthesised by both chemical and biological methods was analysed. Biological method of AgNPs synthesis showed remarkable stability. They were stable for 24 days whereas AgNPs synthesised by chemical method were stable for only 3 days. Also the influence of biosurfactant and chemical surfactant on the stability of AgNPs synthesized by chemical method was analyzed. The biosurfactant showed superior property (19 days) over the chemical surfactant (7 days) in terms of increasing the stability of AgNPs. The use of bacterium for nanoparticles synthesis and the influence of biosurfactant on the stability of AgNPs offer the benefits of eco-friendliness and amenability for large-scale production