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Identification and Molecular Characterisation of Pseudomonas florescence (ADY9 strain) as a Potential Bio-Pesticide

Anamika Rana1, Raj Shekhar Sharma2, and Manjusha Tyagi3*

ADY 9 strain was isolated from rhizospheric soil native to Uttarakhand's high altitude areas to test its efficacy as a promising bio-pesticide. The results indicate that the ADY 9 strain gives positive tests for catalase, chitinolytic activity, gelatine hydrolysis, carbohydrate utilization, protease, xylanase, beta-1,3-glucanase. In addition to this, its effectiveness is managing grey spot disease in turnip was tested in vitro against with fungal inoculums. Outcomes of pot experiment showed the efficacy of formulation reduce disease incidence against control. Along with preventing pathological changes the formulation also induced some visible morphological changes such as increase in the root length, diameter and weight of the turnip in comparison with the control. For confirmation, we have done 16S rRNA sequences, polymerase chain reaction (PCR) amplification and nucleotide sequence analysis using BLAST (NCBI) as Pseudomonas florescence ADY 9 assigned the accession number MT549190 in GenBank. We display and define the genome sequence of Pseudomonas fluorescens strain ADY 9, which consists of a 1,391 bp linear chromosome with GC material analysis, Neighbour Join, and a short minimum evolution graph. Furthermore, the ADY 9 strain's Swiss homology model has been explained in this paper.