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Biodegradation of Reactive Red 11 Azo Dye Using Enterobacter sp. and gamma proteobacterium collected from dye industry

Hemant Kr. Sharma, Kavery Bajpai, Shubhangi Shukla, Reena Kumari, Sujeet K. Singh

Effluent sample collected from a dye industry of Chennai was used as a source for the isolation of 9 morphologically distinct bacteria capable of decolorizing textile dye Reactive red 11 which was purchased from local market in Chennai. Bacterium present in the effluent sample are grown in growth media along with the dye with varying concentration and several colonies were obtained using which decolorization assay was performed using textile dye Reactive red 11to screen for high dye decolorizing bacterial strains. 2 bacterial strains showed high decolorization of Reactive red 11 and these colonies were stored for further use. Genomic DNA of these 2 bacterial strains is isolated and identified as Enterobacter sp. and gamma proteobacteria by 16S rDNA analysis. GC-MS analysis of the degraded product of reactive red 11 these bacterium showed the presence of to 5,8- dihydronapthalen- 2-amine which has a molecular mass of 145.2 and 6-(aminomethyl)-N-benzylnapthalen-2-amine which has a molecular mass of 162.