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Biodegradation of dyes by Basidiomycetes fungi using HPLC and UV-Visible Spectrophotometer

Anuradha Kumari

Basidiomycete’s fungi are used for biodegradation of azo dyes, which is mainly employed in textile, food and pharmaceutical industry. In addition they have some medicinal uses like anti-tumor effect due to their polysaccharide; they can reduce cholesterol level as they produce Lovastatin. They are rich in microelements as well as minerals. Basidiomycete’s fungi can decolorize and degrade Eriochrome black T and Methyl orange. Since these dyes has an ability to depolymerize and mineralize because it cannot degrade lignin with their ligninolytic and extracellular enzymes. The extent of degradation for these dyes by fungi can be evaluated with the help of UV-Visible Spectrophotometer and High Performance Liquid Chromatography. According to our UV Spectroscopic methods, biodegradation of dyes occur by the fungi used in the Kirks medium. Decolorization study showed that the methyl orange dye was removed by more than 50% in 3 days, Eriochrome Balck T (removed by more than 33%) and HPLC analysis determined several degradation products. These results suggest that this fungi body has potential in color removal from textile waste water containing various azo dyes.