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A Computational Study of Interactions of Trimethoprim with Native and Mutant Dihydrofolate Reductase Structures

Vinod Kumar Yata, Anant Saxena, Vinita Sharma, Som Dutt, Ajit Kumar

In this study, we analyzed and compared the interactions of an antibiotic drug, trimethoprim, with native and mutant human Dihydrofolate Reductase DHFR structures by computational methods. We observed that, Ile7, Glu30, Leu22, Val115, Pro61, Phe179 and Tyr182 of native DHFR play an important role in interacting with trimethoprim. Ile7, Glu30, Val112, Trp113, Ile114 and Leu133 of mutant DHFRare identified as key residues in interacting with trimethoprim. This study would help in modifying the structure of trimethoprim for improved affinity of the drug towards the native and mutant structures of human dihydrofolate reductase.